mouse elisa kits  (Elabscience Biotechnology)

Journal: Diabetology & Metabolic Syndrome

Article Title: Irisin regulates integrin αvβ5/FAK/ERK to inhibit neutrophil extracellular traps formation and reduce pancreatic beta-cells pyroptosis in type 2 diabetes mellitus

doi: 10.1186/s13098-025-01852-z

Figure Lengend Snippet: Irisin inhibited the formation of NETs in STZ-induced T2DM mouse model and improved T2DM. A . Glucose tolerance test evaluated the glucose tolerance of T2DM mice treated with Irisin, N = 5; B . ELISA detected the plasma insulin levels of T2DM mice treated with Irisin, N = 5; C - D . ELISA detected the levels of Cit-H3 and MPO in the plasma of T2DM mice treated with Irisin, N = 5; E . ELISA detected the serum Irisin levels of T2DM mice treated with Irisin, N = 3; F - G . Western blotting detected the expression levels of related proteins in pyroptosis and phosphorylation levels of STING and IRE1a, N = 3; H . HE staining detected the morphology of pancreatic tissue in T2DM mice treated with Irisin, N = 3; I . Immunofluorescence staining detected the levels of Cit-H3 and MPO in pancreatic tissue of T2DM mice treated with Irisin, N = 3; ** P < 0.01

Article Snippet: Mouse Irisin ELISA kit (E-EL-M2743), LDH ELISA kit (E-EL-M0419), IL-18 ELISA kit (E-EL-M0730) were purchased from Elabscience.

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Western Blot, Expressing, Phospho-proteomics, Staining, Immunofluorescence

Journal: Diabetology & Metabolic Syndrome

Article Title: Irisin regulates integrin αvβ5/FAK/ERK to inhibit neutrophil extracellular traps formation and reduce pancreatic beta-cells pyroptosis in type 2 diabetes mellitus

doi: 10.1186/s13098-025-01852-z

Figure Lengend Snippet: NETs promoted high glucose-induced Min6 cell pyroptosis, but Irisin could inhibit NETs. A . ELISA detected insulin levels in Min6 culture media under different treatment conditions; B - D . Western blotting detected the expression levels of GSDMD-N and C-caspase-1 in Min6 cells under different treatment conditions; E - F . Flow cytometry detected Min6 cell pyroptosis under different treatment conditions; G . Sytox green staining marked the levels of NETs released under different conditions; N = 3, * P < 0.05, ** P < 0.01

Article Snippet: Mouse Irisin ELISA kit (E-EL-M2743), LDH ELISA kit (E-EL-M0419), IL-18 ELISA kit (E-EL-M0730) were purchased from Elabscience.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Flow Cytometry, Staining

Journal: Diabetology & Metabolic Syndrome

Article Title: Irisin regulates integrin αvβ5/FAK/ERK to inhibit neutrophil extracellular traps formation and reduce pancreatic beta-cells pyroptosis in type 2 diabetes mellitus

doi: 10.1186/s13098-025-01852-z

Figure Lengend Snippet: Pyroptosis induced by NETs was regulated by NLRP1. A . Western blotting was performed to detect the expression of related proteins in pyroptosis in Min6 cells with NLRP1 knocked down; B . ELISA was used to detect the LDH, IL-1βand IL-18; C - D . Flow cytometry was used to detect pyroptosis in NLRP1 knocked down Min6 cells induced by NETs; N = 3, * P < 0.05, ** P < 0.01

Article Snippet: Mouse Irisin ELISA kit (E-EL-M2743), LDH ELISA kit (E-EL-M0419), IL-18 ELISA kit (E-EL-M0730) were purchased from Elabscience.

Techniques: Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Flow Cytometry

Journal: Diabetology & Metabolic Syndrome

Article Title: Irisin regulates integrin αvβ5/FAK/ERK to inhibit neutrophil extracellular traps formation and reduce pancreatic beta-cells pyroptosis in type 2 diabetes mellitus

doi: 10.1186/s13098-025-01852-z

Figure Lengend Snippet: Pyroptosis induced by NETs was regulated by STING/IRE1α. A . ELISA was used to detect the LDH, IL-1β and IL-18; B - C . Western blotting was performed to detect the expression of related proteins in pyroptosis in Min6 cells with C-176 added; D - E . Flow cytometry was used to detect pyroptosis in Min6 cells with C-176 added induced by NETs; N = 3, * P < 0.05, ** P < 0.01

Article Snippet: Mouse Irisin ELISA kit (E-EL-M2743), LDH ELISA kit (E-EL-M0419), IL-18 ELISA kit (E-EL-M0730) were purchased from Elabscience.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Flow Cytometry

Journal: Immunobiology

Article Title: Clostridium butyricum attenuates LPS-induced myocardial injury in septic mice by modulating CD4 + CD25 + FOXP3 + Treg.

doi: 10.1016/j.imbio.2024.152857

Figure Lengend Snippet: Fig. 2. Inflammation in septic myocardial injury mice with marked changes in markers of myocardial injury. (A-C) After the successful construction of a mouse model of sepsis induced myocardial injury, plasma was diluted three-fold and ELISA was used to detect changes in myocardial injury markers and inflammatory markers in the LPS group and control group, respectively (n = 5 per group). The main biomarkers for myocardial injury are cTnI and LDH, while inflammatory biomarkers are IL-1β, IL-6, and TNF-α. A: Changes in cTnI levels between two groups. B: Changes in LDH levels between two groups. C: Changes in IL-1β, IL-6, and TNF-α levels between two groups. D: qPCR assay for mouse heart tissue mRNA levels of inflammatory factors IL-1β, IL-6, and TNF-α between two groups. n represents number of animals, *P represents P < 0.05; **P represents P < 0.01; ***P represents P < 0.001; ****P represents P < 0.0001;

Article Snippet: The standard preparation and related experimental steps were performed according to the instruction manual of the ELISA kits for IL-6 (Sigma-Aldrich #RAB0308), IL-1β (Sigma-Aldrich #RAB0274), TNF-α (Sigma-Aldrich #RAB0477), cTnI (MM #0791 M1), and LDH (Cusabio #CSB-E11723m).

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control

Journal: Immunobiology

Article Title: Clostridium butyricum attenuates LPS-induced myocardial injury in septic mice by modulating CD4 + CD25 + FOXP3 + Treg.

doi: 10.1016/j.imbio.2024.152857

Figure Lengend Snippet: Fig. 5. Clostridium butyricum significantly reduced serum inflammation and myocardial markers in mice with septic myocardial injury. (A-C) After continuous gavage of Clostridium butyricum for 28 days and successful construction of a mouse model of sepsis induced myocardial injury, plasma was diluted three-fold and ELISA was used to detect changes in myocardial injury markers and inflammatory markers in the LPS group and control group, respectively (n = 5 per group). The main biomarkers for myocardial injury are cTnI and LDH, while inflammatory biomarkers are IL-1β, IL-6, and TNF-α. A: Changes in cTnI levels between two groups. B: Changes in LDH levels between two groups. C: Changes in IL-1β, IL-6, and TNF-α levels between two groups. D: qPCR assay for mouse heart tissue mRNA levels of inflammatory factors IL-1β, IL-6, and TNF-α between two groups. n represents number of animals, *P represents P < 0.05; **P represents P < 0.01; ***P represents P < 0.001; ****P represents P < 0.0001;

Article Snippet: The standard preparation and related experimental steps were performed according to the instruction manual of the ELISA kits for IL-6 (Sigma-Aldrich #RAB0308), IL-1β (Sigma-Aldrich #RAB0274), TNF-α (Sigma-Aldrich #RAB0477), cTnI (MM #0791 M1), and LDH (Cusabio #CSB-E11723m).

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control

Journal: Immunobiology

Article Title: Clostridium butyricum attenuates LPS-induced myocardial injury in septic mice by modulating CD4 + CD25 + FOXP3 + Treg.

doi: 10.1016/j.imbio.2024.152857

Figure Lengend Snippet: Fig. 2. Inflammation in septic myocardial injury mice with marked changes in markers of myocardial injury. (A-C) After the successful construction of a mouse model of sepsis induced myocardial injury, plasma was diluted three-fold and ELISA was used to detect changes in myocardial injury markers and inflammatory markers in the LPS group and control group, respectively (n = 5 per group). The main biomarkers for myocardial injury are cTnI and LDH, while inflammatory biomarkers are IL-1β, IL-6, and TNF-α. A: Changes in cTnI levels between two groups. B: Changes in LDH levels between two groups. C: Changes in IL-1β, IL-6, and TNF-α levels between two groups. D: qPCR assay for mouse heart tissue mRNA levels of inflammatory factors IL-1β, IL-6, and TNF-α between two groups. n represents number of animals, *P represents P < 0.05; **P represents P < 0.01; ***P represents P < 0.001; ****P represents P < 0.0001;

Article Snippet: The standard preparation and related experimental steps were performed according to the instruction manual of the ELISA kits for IL-6 (Sigma-Aldrich #RAB0308), IL-1β (Sigma-Aldrich #RAB0274), TNF-α (Sigma-Aldrich #RAB0477), cTnI (MM #0791 M1), and LDH (Cusabio #CSB-E11723m).

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control

Journal: Immunobiology

Article Title: Clostridium butyricum attenuates LPS-induced myocardial injury in septic mice by modulating CD4 + CD25 + FOXP3 + Treg.

doi: 10.1016/j.imbio.2024.152857

Figure Lengend Snippet: Fig. 5. Clostridium butyricum significantly reduced serum inflammation and myocardial markers in mice with septic myocardial injury. (A-C) After continuous gavage of Clostridium butyricum for 28 days and successful construction of a mouse model of sepsis induced myocardial injury, plasma was diluted three-fold and ELISA was used to detect changes in myocardial injury markers and inflammatory markers in the LPS group and control group, respectively (n = 5 per group). The main biomarkers for myocardial injury are cTnI and LDH, while inflammatory biomarkers are IL-1β, IL-6, and TNF-α. A: Changes in cTnI levels between two groups. B: Changes in LDH levels between two groups. C: Changes in IL-1β, IL-6, and TNF-α levels between two groups. D: qPCR assay for mouse heart tissue mRNA levels of inflammatory factors IL-1β, IL-6, and TNF-α between two groups. n represents number of animals, *P represents P < 0.05; **P represents P < 0.01; ***P represents P < 0.001; ****P represents P < 0.0001;

Article Snippet: The standard preparation and related experimental steps were performed according to the instruction manual of the ELISA kits for IL-6 (Sigma-Aldrich #RAB0308), IL-1β (Sigma-Aldrich #RAB0274), TNF-α (Sigma-Aldrich #RAB0477), cTnI (MM #0791 M1), and LDH (Cusabio #CSB-E11723m).

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control